Molecular Biology And Biotechnology ( Biotech ) - 910 Words

CARBON SOURCE The lignocellulosic hydrolysate will be prepared by the National Institute of Molecular Biology and Biotechnology (BIOTECH, UPLB) from sweet sorghum bagasse subjected to pretreatment followed by sulfuric acid (0.75 wt% and pH 1.1) hydrolysis at 100∘C. BACTERIAL STRAIN Cupriavidus necator is a bacterial strain that is commonly used in accumulating poly-3-hydroxybutyrate (PHB), the most common type of PHA. It is most effective when the amount of carbon source present is in excess compared to the other essential nutrients in the medium like nitrogen, phosphorus or oxygen (Lopez-Abelairas, Garcia- Torreiro, Lu-Chau, Lema, Steinbuchel, 2015). This bacterial strain will be pre-cultured from within 24 hours in a 500 mL Erlenmeyer flask under 30∘C and 150 revolutions per minute (rpm) without the addition of other carbon source (Martinez, Bertin, Scoma, Rebecchi, Braunegg, Fava, 2015). The C. necator culture will be stored at -80∘C in a 2 mL cryovial containing 300  µL of glycerol with 20 grams per liter (g/L) of xylose (Cesario, Raposo, de Almeida, van Keulen, Ferreira, da Fonseca, 2014). In this experiment, â€Å"dual-phase strategy† will be performed. This will employ that two different media will be prepared for the first an d the second process. The first process will involve the cell growth or the balanced growth phase. On the other hand, the second process will involve the accumulation of PHA. Both media that will be prepared will limit the ammonia (NH3)Show MoreRelatedA Research Study On Microbial Ecology : Biogeography, Disease Dispersal And Potential Source Of New Biotech Products1006 Words   |  5 Pagesabout biological sciences, especially in topics covering microbiology and molecular biology. 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